No.66 (March,2004)
No.66 (March,2004)
A－372 Nobukazu SATOH, Takashi SUGIMOTO
Effects of feeding intervals on the growth of the juveniles of Brown Sole Pleuronectes herzensteini.
This experiment was conducted to evaluate the growth, the feed conversion efficiency （total coefficient growth / total estimated uptake×100）, the time-related changes of the uptakes during rearing periods, the rate of decreasing body weight by starvation at three different feeding intervals; everyday（group A）, every other day（group B）and every third day（group C） of the juvenile brown sole Pleuronectes herzensteini.
The growth of group A or B was greater than that of group C, and there was not significantly difference in the growth of group A and B. On the other hand, in the feed conversion efficiency, group C was the first value among experimental groups, group B was the second value among them. The time-related changes of the uptakes for rearing periods of group B or C were usually found to be more than those of group A. In the rate of decreasing body weight by starvation, group B or C was remarkably less than group A.
These results show that it is effective technique for the culture of the juvenile of brown sole to feed every other day. And in this experiment we showed that it was possible to decrease the amount of 25% in the total amount of feeding by using the way.
This experiment was conducted to evaluate the growth, the feed conversion efficiency （total coefficient growth / total estimated uptake×100）, the time-related changes of the uptakes during rearing periods, the rate of decreasing body weight by starvation at three different feeding intervals; everyday（group A）, every other day（group B）and every third day（group C） of the juvenile brown sole Pleuronectes herzensteini.
The growth of group A or B was greater than that of group C, and there was not significantly difference in the growth of group A and B. On the other hand, in the feed conversion efficiency, group C was the first value among experimental groups, group B was the second value among them. The time-related changes of the uptakes for rearing periods of group B or C were usually found to be more than those of group A. In the rate of decreasing body weight by starvation, group B or C was remarkably less than group A.
These results show that it is effective technique for the culture of the juvenile of brown sole to feed every other day. And in this experiment we showed that it was possible to decrease the amount of 25% in the total amount of feeding by using the way.
A－373 Shingo ITO, Kouki MIURA, Nobukazu SATOH
Study of pathogenicity of Aeromonas salmonicida like bacteria from brown sole（Pleuronectes herzensteini）to other flatfish species
Isolated bacteria from kidneys of dead brown soles in a tank were similar to A．salmonicida sub.
salmonicida. Intraperitoneal injection with 105～7CFU per fish of isolated bacteria killed all of 5 injected fish, injection with 102～4CFU per fish killed four fish, and injection with 101CFU per fish killed one fish; immersion with 107CFU per L for 1 hour killed all tested fries. Therefore, the strain of this study was obligate pathogen for brown sole. Injection with 107CFU per fish to Japanese flounders killed 10 out of 10 fish and with 105CFU per fish killed four fish, but injection with 103 CFU per fish killed no fish.
Intraperitoneal injection with 107CFU per fish also killed 8 out of 10 barfin flounder, but did not kill with under 105CFU per fish of bacteria. The strain used in this study has the capability to kill not only brown sole but also Japanese flounder and barfin flounder.
Isolated bacteria from kidneys of dead brown soles in a tank were similar to A．salmonicida sub.
salmonicida. Intraperitoneal injection with 105～7CFU per fish of isolated bacteria killed all of 5 injected fish, injection with 102～4CFU per fish killed four fish, and injection with 101CFU per fish killed one fish; immersion with 107CFU per L for 1 hour killed all tested fries. Therefore, the strain of this study was obligate pathogen for brown sole. Injection with 107CFU per fish to Japanese flounders killed 10 out of 10 fish and with 105CFU per fish killed four fish, but injection with 103 CFU per fish killed no fish.
Intraperitoneal injection with 107CFU per fish also killed 8 out of 10 barfin flounder, but did not kill with under 105CFU per fish of bacteria. The strain used in this study has the capability to kill not only brown sole but also Japanese flounder and barfin flounder.
A－374 Yoshiyuki TAKAYA, Taihei MATSUDA, Hidetsugu YOSHIDA
Validation of age determination from otoliths of young barfin flounder（Verasper moseri）.（Short Paper）
A－375 Yasufumi HADA, Kunio TAKASHIMA, Yuji MACHIGUCHI
On the predation of Inclytum whelk Buccinum inclytum and Oregon triton Fusitriton oregonensis by sea star Asterias amurensis（Short Paper）