ＢＳＥに関する研究

・北海道農業試験会議　成績会議資料

非定型BSE感染牛の歩様と行動量の変化（H25)
<成績概要書　PDF>

試験管内増幅法を用いたBSE発症牛の唾液からのﾌﾟﾘｵﾝ高感度検出技術（H24）
<成績概要書　PDF>

非定型BSE感染牛におけるプリオンの病原性と体内分布(H23)
<成績概要書　PDF>

BSE脳内感染実験牛のプリオン體內分布(H19)
<成績概要書>

BSE疑似患畜の経過観察と脳内接種法の確立(H16)
<成績概要書 PDF>

・論文

2013
Ohtsuka H, Fukuda S, Kudo K, Tomioka M, Koiwa M, Kimura K.(2013)
Changes in leukocyte populations of cows with milk fever or displaced abomasum after calving.
Can J Vet Res.77(3)226-230

Okada H, Murayama Y, Shimozaki N, Yoshioka M, Masujin K, Imamura M, Iwamaru Y, Matsuura Y, Miyazawa K, Fukuda S, Yokoyama T, Mohri S.
Prion in saliva of bovine spongiform encephalopathy-infected cattle.
Emerg Infect Dis.18(12)2091-2092

2012
Fukuda S, Onoe S, Nikaido S, Fujii K, Kageyama S, Iwamaru Y, Imamura M, Masujin K, Matsuura Y, Shimizu Y, Kasai K, Yoshioka M, Murayama Y, Mohri S, Yokoyama T, Okada H. (2012) Neuroanatomical distribution of disease-associated prion protein in experimental bovine spongiform encephalopathy in cattle after intracerebral inoculation.Jpn J Infect Dis. 65:37-44

2011
Okada H, Iwamaru Y, Fukuda S, Yokoyama T, Mohri S. (2011) Detection of disease-associated prion protein in the optic nerve and the adrenal gland of cattle with bovine spongiform encephalopathy by using highly sensitive immunolabeling procedures. 60:290-300.

Okada H, Iwamaru Y, Kakizaki M, Masujin K, Imamura M, Fukuda S, Matsuura Y, Shimizu Y, Kasai K, Mohri S, Yokoyama T. (2011) Properties of L-type bovine spongiform encephalopathy in intraspecies passages. Vet Pathol.

Fukuda S, Okada H, Arai S, Yokoyama T, Mohri S. (2011) Neuropathological changes in auditory brainstem nuclei in cattle with experimentally induced bovine spongiform encephalopathy. J Comp Pathol. 145:302-307.

Okada H, Iwamaru Y, Imamura M, Masujin K, Matsuura Y, Shimizu Y, Kasai K, Takata M, Fukuda S, Nikaido S, Fujii K, Onoe S, Mohri S, Yokoyama T. (2011) Neuroanatomical Distribution of Disease-Associated Prion protein in Cases of Bovine Spongiform Encephalopathy Detected by Fallen Stock Surveillance in Japan. J Vet Med Sci. 73:1465-1471.

2010
Murayama Y, Yoshioka M, Masujin K, Okada H, Iwamaru Y, Imamura M, Matsuura Y, Fukuda S, Onoe S, Yokoyama T, Mohri S. (2010) Sulfated dextrans enhance in vitro amplification of bovine spongiform encephalopathy PrP(Sc) and enable ultrasensitive detection of bovine PrP(Sc). PLoS One. 5. pii: e13152.

Iwamaru Y, Imamura M, Matsuura Y, Masujin K, Shimizu Y, Shu Y, Kurachi M, Kasai K, Murayama Y, Fukuda S, Onoe S, Hagiwara K, Yamakawa Y, Sata T, Mohri S, Okada H, Yokoyama T. (2010) Accumulation of L-type bovine prions in peripheral nerve tissues. Emerg Infect Dis.... 16:1151-154.

2009
Fukuda S, Iwamaru Y, Imamura M, Masujin K, Shimizu Y, Matsuura Y, Shu Y, Kurachi M, Kasai K, Murayama Y, Onoe S, Hagiwara K, Sata T, Mohri S, Yokoyama T, Okada H. (2009) Intraspecies transmission of L-type-like Bovine Spongiform Encephalopathy detected in Japan. Microbiol Immunol. 53(12):704-7.

Arai S, Matsui Y, Fukuda S, Okada H, Onoe S. (2009) Brainstem auditory evoked potentials in experimentally-induced bovine spongiform encephalopathy. Res Vet Sci.87(1):111-4.

・学会発表

2013
Asian Pasific Prion Symposium
Fukuda S, Fujii T, Kageyama S, Okada H,
Detection specificity of salivary BSE PrPSc by PMCA.

北海道産業動物獣医学会
福田茂夫、藤井貴志、陰山聡一、岡田洋之、横山隆、村山裕一
PMCA法を用いたBSE発症牛の唾液からのPrPSc検出技術

2012
第154回日本獣医学会
藤井貴志、福田茂夫、陰山聡一、新井鐘蔵、平山博樹、内藤　学、南橋　昭
携帯型の牛用脳幹機能測定装置を用いた黒毛和種牛の聴性脳幹誘発電位の測定

APPS 2012
Fukuda S, Fujii T, Kageyama S, Okada H, Yokoyama T, Murayama Y.
Preclinical detection of PrPSc in eyes of BSE-affected cattle.

2011
PRION 2011
Fukuda S, Okada H, Arai S, Kageyama S, Yokoyama T, Mohri S.
Neuropathological changes in auditory brainstem nuclei in cattle with experimentally induced bovine spongiform encephalopathy.

家畜感染症学会2011
福田茂夫、岡田洋之、新井鐘蔵、陰山聡一、横山隆、毛利資郎
脳内接種BSEプリオン感染牛の聴覚神経路病変

2010
Asia-Oceania Symposium on Prion Disease 2010
Fukuda, S, Iwamaru, Y, Masujin, K, Onoe, S, Kageyama, S, Minamihashi, A, Kusakari N, Okada, H, Yokoyama, T .
Intraspecies transmission of L-type bovine spongiform encephalopathy detected in Japan.

家畜繁殖に関する研究

・北海道農業試験会議　成績会議資料

低ランク胚移植における単為発生胚の共移植による妊娠認識増強効果(H25）
<成績概要書　PDF>

追い移植による乳牛の長期不受胎対策の効果検証（H24）
<成績概要書　PDF>

ドナー牛の過剰胚卵処理における卵巣反応性の予測技術 (H23)
<成績概要書　PDF>

牛の分娩誘起が胎盤節のアポトーシスの発生に及ぼす影響 (H23)
<成績概要書　PDF>

・論文

2013
Wasielak M, Fujii T, Osaki T, Hashizume T, Bogacki M, Sawai K. (2013)
Transcript abundance and apoptosis in day-7 porcine blastocyst cultured with exogenous IGF-I.Reprod Biol.13(1)：58-65

Fujii T, Sakurai N, Osaki T, Iwagami G, Hirayama H, MInamihashi A, Hashizume T, Sawai K. (2013)
Changes in the Expression Patterns of the Genes Involved in the Segregation and Function of Inner Cell Mass and Trophectoderm Lineages During Porcine Preimplantation Development. J Reprod Dev.59(2)：151-158

Hirayama H, Kageyama S, Moriyasu S, Sawai K, Minamihashi A. (2013)
Embryo sexing and sex chromosomal chimerism analysis by loop-mediated isothermal amplification in cattle and water buffaloes. J Reprod Dev.59(4)：321-326

Sakurai N, Fujii T, Hashizume T, Sawai K. (2013)
Effects of downregulating Oct-4 transcript by RNA interference on earl development of porcine embryos. J Reprod Dev.59(4)：353-360

2012
Hirayama H, Usizawa K, Takahashi T, Sawai K, Moriyasu S, Kageyama S, Miura R, Matsui M, Fukuda S, Naito A, Fujii T, Minamihashi A. (2012)
Difference in Apoptotic Status in the Bovine Placentome between Spontaneous and Induced Parturition. J Reprod Dev.

Kageyama S, Hirayama H. (2012)
Sexing of Bovine Preimplantation Embryos using Loop-mediated Isothermal Amplification (LAMP). J Mamm Ova Res.

Hirayama H, Kageyama S, Naito A, Fukuda S, Fujii T, Minamihashi A. (2012)
Prediction of superovulation response in Japanese black cattle using ultrasound test, plasma Anti-Mullerian hormone concentoration and polymorphism in ionotropic glutamate receptor AMPA1/GRIA1. J Reprod Dev.

Sawai K, Fujii T, Hirayama H, Hashizume T, Minamihashi A (2012)
Epigenetic status and full-term development of bovine cloned embryos treated with trichostatin A.J Reprod Dev.

2011
Sawai K, Takahashi M, Fujii T, Moriyasu S, Hirayama H, Minamihashi A, Hashizume T, Onoe S. (2011)
DNA methylation status of bovine blastocyst embryos obtained from various procedures. J Reprod Dev. 57:236-241.

Hirayama H, Sawai K, Hirayama M, Hirai T, Kageyama S, Onoe S, Minamihashi A, Moriyasu S. (2011)
Prepartum maternal plasma glucose concentrations and placental glucose transporter mRNA expression in cows carrying somatic cell clone fetuses. J Reprod Dev. 57:57-61.

2010
Fujii T, Moriyasu S, Hirayama H, Hashizume T, Sawai K. (2010)
Aberrant expression patterns of genes involved in segregation of inner cell mass and trophectoderm lineages in bovine embryos derived from somatic cell nuclear transfer. Cell Reprogram. 12:617-625.

Bhuiyan MM, Suzuki Y, Watanabe H, Lee E, Hirayama H, Matsuoka K, Fujise Y, Ishikawa H, Ohsumi S, Fukui Y. (2010)
Production of Sei whale (Balaenoptera borealis) cloned embryos by inter- and intra-species somatic cell nuclear transfer. J Reprod Dev. 56:131-139

Hirayama H, Kageyama S, Moriyasu S, Sawai K, Onoe S, Minamihashi A. (2010)
[タイトル不明]

・学会発表

2013
日本繁殖生物学会
藤井貴志、佐久間正樹、平山博樹、澤井和彦、澤井一樹
牛胚の発生段階および遺伝子発現解析

2012
日本繁殖生物学会
平山博樹、藤井貴志、澤井和彦、澤井一樹
牛胚の性判別および性染色体キメラ解析

2011
日本繁殖生物学会
澤井一樹、藤井貴志、平山博樹、澤井和彦
牛胚のDNAメチル化状態解析